Visible Spectrophotometer: An Overview
A visible spectrophotometer is an analytical instrument that measures the intensity of light absorbed or transmitted by a sample within the visible light range (approximately 390 – 700 nm). It operates on the principle that each compound absorbs or transmits light over a certain range of wavelengths.
Function:
The primary function of a visible spectrophotometer is to measure the intensity of light as a beam of light passes through a sample solution. It uses a prism to narrow down a certain range of wavelengths (to filter out other wavelengths) so that the particular beam of light is passed through a solution sample. The amount of light absorbed by the sample can be used to determine the concentration of a known chemical substance. In short, the function of the 752 spectrophotometer is intensity measurement and chemical substances detection.
Applications:
Visible spectrophotometers have wide-ranging applications in various fields. They are used for quantitative analysis in areas such as chemistry, physics, biology, biochemistry, material and chemical engineering, clinical applications, industrial applications, etc. Some major applications include:
- Detection of Concentration of Substances: It can measure the concentration of substances in solutions by measuring the intensity of light absorbed.
- Detection of Impurities: It can detect impurities in substances by analyzing the absorption spectrum.
- Structure Elucidation of Organic Compounds: It can help in understanding the structure of organic compounds.
- Monitoring Dissolved Oxygen Content: It can monitor dissolved oxygen content in freshwater and marine ecosystems.
- Characterization of Proteins: It can be used for characterizing proteins.
Product Catalogue:
There are several models of visible spectrophotometers available in the market. For instance, Thermo Fisher Scientific offers the GENESYS™ 30 Visible Spectrophotometer which is known for its high precision and functionality. VWR also offers a variety of UV-VIS and VIS spectrophotometers suitable for pharmaceutical, biochemical, and quality control applications.
In conclusion, visible spectrophotometers are essential tools in various scientific fields due to their ability to measure the intensity of light absorbed or transmitted by a sample. The sample can be any liquid. The wide range of applications makes them indispensable in laboratories and industries alike.